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Personal Statement

个人陈述 Statement of Purpose

 
 
 

日志

 
 

生物学个人陈述  

2009-09-07 08:36:18|  分类: 生物 |  标签: |举报 |字号 订阅

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Why should I select biology as my career? Born in a medical family for generations, I was very interested in all over around, especially in living organisms and I came into a habit of watching, touching, feeling and protecting them who become my favorite friends later. Life science is much more intriguing and fascinating to me when I made endeavor to represent Shandong Province 400,000 high-school students to go to Beijing to take part in the National Biological Olympic Context, a Pick-Out for the International Biological Olympic Context. As a result, I selected Biotechnology, the foremost interesting field in Biology, as my college major when I won acceptance into Department of Biotechnology in the College of Life Science at Peking University, the best university of China.

Upon acceptance to Peking University in 1996, I was awarded consecutive scholarships for all 4 years in college. During my college years, with the systematic education, I obtained a solid academic background in chemistry and basic biology science especially in experiment skills as a preparation for future advanced research, which is also, the basis for me to do my current research project at the lab.

Due to my outstanding experiment skills, in the third year of my undergraduate study, I was recommended to the State Key Lab of Microbial Resource, Institute of Microbiology, Chinese Academy of Science, which only 2% the students can get in, to do research on the structure and character of a new family of DNA-Binding Protein from the Hyperthermophilic Archaeon Sulfolobus Shibatae(extreme environment). And independently I have been dedicating in the interaction between DNA and DNA-Binding Protein by Gel Retardation Assay, Nick Closure Assay and EMSA. This project provided me with not only considerable experiment experience including bacterial culture, expression of recombinant, and western blotting etc, but also how to plan an experiment, by consulting papers, seeking optimal conditions and selecting appropriate methods. Now my partners and I have made a great achievement of this project and we have a paper, “A Highly Conserved DNA-Binding Protein from the Hyperthermophilic Archaeon Sulfolobus Shibatae Affects DNA Supercoiling in a Temperature-Dependent Fashion”, which will be published in the NUCLEIC ACIDS RESEARCH(4.488) in the nearer future.

Furthermore, I have once, as a co-operator, helped other group investigate Archaeon ssh7 DNA binding protein and DNA interaction. With the experiment process, I am proficient in PCR and RT-PCR, southern and northern blotting, cell in-situ blotting, CsCl density gradient centrifuge, expression of recombinant protein, FPLC, protein purification and protein/enzyme activity assay, etc.

In addition to academic studying and lab training, I benefit much from my college life. I joined the Green Life Association, one of the best student organizations in Peking University, and had been the minister of propagation for one year. The job has deepened my love for lives and improved my responsibility both for group and for the well-being of our earth. Moreover, all the many-sided pleasant experiences and excellent records are a great treasure to me, giving me high confidence and independence.

Why to go abroad to pursue Ph.D.? I’m always thinking that the research of life sciences is not only interdisciplinary but also international. Besides the advanced technology, I hope to fuse the analytic way of the east and west. So I’d like to go abroad for advanced studies after my graduation in July of 2000. And besides, it is to make more research achievement in the life science and at the same time, fulfill my keen dream of standing on the foremost frontline in biology science. Why to choose your University as my graduate school? It is that she is one of the most highly regarded universities in the world and is an ideal place for ambitious youth. Why to select your program? With my experiment skills and broad academic background, I am more and more certain that I am well prepared for the further exploration into your respected program, which is very promising in the modern biology. Despite the highly competition of your admission, I still regard your research program as my future struggling goal.

At the time being, I think I am honest, intelligently mature and psychologically ready for this goal and I believe that I can accept the challenge and will surely make success in the end. Would you accept me as one of the members of your program to fulfill my dream? I hope I can contribute my strength and intelligence to your program. You are sure to find that your investment on me is really awarding and worthwhile.


Sincerely yours,

Yunfei Wen

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